Komuro T, Zhou D S
Department of Basic Human Sciences, School of Human Sciences, Waseda University, Saitama, Japan.
J Auton Nerv Syst. 1996 Nov 6;61(2):169-74. doi: 10.1016/s0165-1838(96)00078-1.
Interstitial cells of Cajal (ICC) of the guinea-pig small intestine were studied with whole-mount preparations by using the zinc iodide-osmic acid method (ZIO) and immunohistochemistry for vimentin and c-kit receptor tyrosine kinase, and by electron microscopy. The myenteric ICC visualized with ZIO staining are immunopositive to both anti-c-kit antibody (ACK-2) and anti-vimentin antibody (V9), and constitute an independent cellular network from the myenteric plexus. Those cells are characterized by many mitochondria, abundant intermediate filaments, and surface cell membranes not covered with a basal lamina. They are connected with each other by gap junctions at tips of the cytoplasmic processes. It is concluded that the myenteric ICC of the guinea-pig intestine are fibroblast-like cells and that they correspond to the c-kit expressing cells regarded as the intestinal pacemaker.
采用碘化锌-锇酸法（ZIO）、波形蛋白和c-kit受体酪氨酸激酶免疫组织化学方法以及电子显微镜技术，对豚鼠小肠的Cajal间质细胞（ICC）进行了整装标本研究。经ZIO染色显示的肌间ICC对抗c-kit抗体（ACK-2）和抗波形蛋白抗体（V9）均呈免疫阳性，且构成了一个独立于肌间神经丛的细胞网络。这些细胞的特征为含有许多线粒体、丰富的中间丝以及未被基膜覆盖的表面细胞膜。它们在细胞质突起的末端通过缝隙连接相互连接。结论是，豚鼠肠道的肌间ICC是成纤维细胞样细胞，且它们相当于被视为肠道起搏器的c-kit表达细胞。
