Brandt B, Junker R, Griwatz C, Heidl S, Brinkmann O, Semjonow A, Assmann G, Zänker K S
Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelms-Universität, Münster, Germany.
Cancer Res. 1996 Oct 15;56(20):4556-61.
The detection of blood-borne prostate cancer (PCA) cells may help with clinical staging and the further understanding of PCA metastases. We discovered prostate-specific antigen (PSA)-positive stained but not PSA mRNA-expressing blood cells by means of cell sorting and PSA reverse transcription-PCR in patients. Therefore, we developed a cytokeratin immunomagnetic method to isolate PSA-positive epithelial cells from the circulating blood of PCA patients. We obtained blood-borne single cells from 6 of 10 PCA patients and clustered cells from 8 of 10 PCA patients. Patients with benign prostate hyperplasia tested negative for cell clusters. The reported isolation method yielded prostate-derived cells or clusters of them from PCA-diagnosed patients.
检测血行播散的前列腺癌细胞可能有助于临床分期以及对前列腺癌转移的进一步了解。我们通过细胞分选和前列腺特异性抗原（PSA）逆转录聚合酶链反应（RT-PCR）在患者中发现了PSA染色阳性但不表达PSA mRNA的血细胞。因此，我们开发了一种细胞角蛋白免疫磁珠法，从前列腺癌患者的循环血液中分离PSA阳性上皮细胞。我们从10例前列腺癌患者中的6例获得了血行播散的单个细胞，并从10例前列腺癌患者中的8例获得了聚集细胞。良性前列腺增生患者的细胞团检测为阴性。所报道的分离方法从前列腺癌确诊患者中获得了前列腺来源的细胞或细胞团。
