Bhakoo K K, Williams S R, Florian C L, Land H, Noble M D
The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, United Kingdom.
Cancer Res. 1996 Oct 15;56(20):4630-5.
Analysis of transformed, immortalized, and primary rat Schwann cells by high-resolution proton nuclear magnetic resonance spectroscopy reveals that immortalization of Schwann cells (by SV40 large T antigen) induced a decrease in sn-glycero-3-phosphocholine (GPCho), whereas H-ras alone, which is known to cause growth arrest in these cells, induced a marked increase in GPCho and a decrease in phosphocholine (PCho). An increase of PCho was found only in cells fully transformed by both oncogenes together. Moreover, we examined 11 human tumor cell lines, all of which expressed a PCho:GPCho ratio similar to that of fully transformed rat Schwann cells. Importantly, neither the absolute levels of PCho nor the ratio of PCho:GPCho were correlated with the rate of cell division across a range of normal (primary cultures) and transformed cells. Thus, raised PCho:GPCho ratios may serve as an indicator of multiple oncogenic lesions and malignancy in noninvasive tumor investigations.
通过高分辨率质子核磁共振波谱对转化的、永生化的和原代大鼠雪旺细胞进行分析发现，雪旺细胞永生化（通过SV40大T抗原）导致sn-甘油-3-磷酸胆碱（GPCho）减少，而单独的H-ras已知会导致这些细胞生长停滞，它会导致GPCho显著增加和磷酸胆碱（PCho）减少。仅在由两种癌基因共同完全转化的细胞中发现PCho增加。此外，我们检测了11种人类肿瘤细胞系，所有这些细胞系表达的PCho:GPCho比值与完全转化的大鼠雪旺细胞相似。重要的是，在一系列正常（原代培养物）和转化细胞中，PCho的绝对水平和PCho:GPCho比值均与细胞分裂速率无关。因此，升高的PCho:GPCho比值可能在非侵入性肿瘤研究中作为多种致癌病变和恶性肿瘤的一个指标。
