Florian C L, Preece N E, Bhakoo K K, Williams S R, Noble M D
Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, United Kingdom.
Cancer Res. 1995 Jan 15;55(2):420-7.
We compared the properties of six human meningiomas with normal rat meningeal cells using cell culture techniques, high resolution in vitro 1H-NMR (nuclear magnetic resonance) spectroscopy, and chromatographic analysis. Cell cultures were immunocytochemically characterized at all stages with specific antibodies. Quantitative and qualitative metabolite assessments in cell extracts were obtained from 1H-NMR spectra and chromatographic analysis. Human meningioma cells expressed a characteristic spectrum of metabolites including free amino acids, compounds related to membrane phospholipid metabolism, energy metabolites, and other intermediary products. These spectral characteristics, although different in some respects, were strikingly similar to the ones of rat meningeal cells. Particularly, several metabolites that allow discrimination between meningeal cells and other cell types of the central nervous system were preserved in meningiomas. These similarities suggest that the regulation of intracellular levels of such metabolites is so intrinsic to the identity of cell type as to be conserved across species and through transformation. Additionally, human meningioma cultures expressed some spectroscopic characteristics that enabled them to be clearly distinguished from primary rat meningeal cultures. Thus, human meningiomas may be both specifically recognizable by 1H-NMR spectroscopy and also distinguishable from normal rat meningeal tissue. Our results raise the eventual possibility of using NMR in the noninvasive diagnosis of brain tumors in vivo.
我们运用细胞培养技术、高分辨率体外¹H-NMR（核磁共振）光谱法以及色谱分析，对6例人类脑膜瘤的特性与正常大鼠脑膜细胞进行了比较。在各个阶段，均使用特异性抗体通过免疫细胞化学方法对细胞培养物进行表征。从¹H-NMR光谱和色谱分析中获取细胞提取物中代谢物的定量和定性评估结果。人类脑膜瘤细胞表达出一种特征性的代谢物谱，包括游离氨基酸、与膜磷脂代谢相关的化合物、能量代谢物以及其他中间产物。这些光谱特征尽管在某些方面存在差异，但与大鼠脑膜细胞的光谱特征极为相似。特别值得一提的是，脑膜瘤中保留了几种能够区分脑膜细胞与中枢神经系统其他细胞类型的代谢物。这些相似性表明，此类代谢物细胞内水平的调节对于细胞类型的特性而言是如此内在，以至于在物种间以及细胞转化过程中得以保守。此外，人类脑膜瘤培养物表现出一些光谱特征，使其能够与原代大鼠脑膜培养物明显区分开来。因此，人类脑膜瘤既可以通过¹H-NMR光谱法被特异性识别，也能够与正常大鼠脑膜组织区分开来。我们的研究结果提出了在体内对脑肿瘤进行无创诊断时最终使用核磁共振的可能性。
