Ishikubo A, Yashiro M, Komiyama M
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Nucleic Acids Symp Ser. 1995(34):85-6.
Dinuclear Zn(II) and La(III) complexes with TPHP efficiently hydrolyze a dinucleotide, ApA, under mild conditions (TPHP = N, N, N', N'-tetrakis[(2-pyridyl)-methyl]-2-hydroxy-1,3-diaminopropane). [Zn2(TPHP)]3+ hydrolyzes ApA with an extremely high activity; The pseudo-first-order rate constant is 8.4 x 10(-4) h-1 at pH 7, 50 degrees C when [[Zn2 (TPHP)]3+] = 2.5 mM. Free Zn(II) ion shows no hydrolysis activity under the conditions. Enormous acceleration of the hydrolysis by the dinuclear complex formation was also observed for La(III) ion. Its activity for the ApA hydrolysis is 100-fold greater than that of free La(III) ion. These dinuclear complexes are promising for the active sites of artificial ribonucleases.
含TPHP的双核锌（II）和镧（III）配合物在温和条件下能有效水解二核苷酸ApA（TPHP = N，N，N'，N'-四[（2-吡啶基）甲基]-2-羟基-1，3-二氨基丙烷）。[Zn2(TPHP)]3+以极高的活性水解ApA；在pH 7、50℃且[[Zn2(TPHP)]3+] = 2.5 mM时，准一级速率常数为8.4×10(-4) h-1。在该条件下，游离的锌（II）离子无水解活性。对于镧（III）离子，双核配合物的形成也极大地加速了水解。其水解ApA的活性比游离镧（III）离子高100倍。这些双核配合物有望成为人工核糖核酸酶的活性位点。
