Mamdani B H, Shaykh M M, Evenson M A, Dunea G
Int J Artif Organs. 1979 Jul;2(4):187-91.
In order to characterize the spectrum of possibly toxic retention compounds in uremia we have developed a simple reproducible method of separating fractions of uremic serum by Sephadex G-15 column chromatography. This technique, which requires no prior deproteinisation and is carried out at ambient temperatures, allowed the separation of uremic serum into several well defined fractions. Subsequent thin layer chromatography (TLC) showed that each peak represented a mixture of peptides, and that there were qualitative and quantitative differences between the plasma of normal and uremic patients as well as between patients with acute renal failure and chronic renal failure.
为了描述尿毒症中可能具有毒性的潴留化合物的谱，我们开发了一种简单且可重复的方法，通过葡聚糖G - 15柱色谱法分离尿毒症血清组分。该技术无需预先进行脱蛋白处理，且在室温下进行，能够将尿毒症血清分离成几个界限分明的组分。随后的薄层色谱法（TLC）表明，每个峰代表肽的混合物，并且正常人和尿毒症患者的血浆之间以及急性肾衰竭和慢性肾衰竭患者之间存在定性和定量差异。
