Sadykov A S, Salikhov Sh I, Mikhamedieva Sh G, Kasymov Sh K, Pak N P
Biull Eksp Biol Med. 1984 Aug;98(8):245-7.
A homogenic peptide (m. w. 2100 dalton) was isolated from blood serum of patients suffering from renal failure by gel chromatography on a Sephadex G-25 column and by partition chromatography on a column with cellulose MN-300. The peptide was not detectable in the blood of healthy donors. The amino acid composition of the peptide was studied. To obtain an immunosorbent, the peptide was conjugated by the bifunctional reagent, glutaric aldehyde. An optimal conjugation variant promoting the formation of conjugates with a molecular weight of 60000 dalton was developed. The molecular weight of the conjugates was measured by gel chromatography on a Sephadex column G-50 and with the aid of disc electrophoresis in polyacrylamide gel.
通过在Sephadex G - 25柱上进行凝胶色谱法以及在装有纤维素MN - 300的柱上进行分配色谱法，从肾衰竭患者的血清中分离出一种同源肽（分子量2100道尔顿）。在健康供体的血液中未检测到该肽。对该肽的氨基酸组成进行了研究。为了获得免疫吸附剂，用双功能试剂戊二醛将该肽进行偶联。开发出了一种促进形成分子量为60000道尔顿的偶联物的最佳偶联变体。通过在Sephadex G - 50柱上进行凝胶色谱法以及借助聚丙烯酰胺凝胶中的圆盘电泳来测定偶联物的分子量。
