Baumann K I, Chan E, Halata Z, Senok S S, Yung W H
Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Neurosci Lett. 1996 Jul 26;213(1):1-4. doi: 10.1016/0304-3940(96)12813-5.
Sinus hairs were isolated from rats and examined in an isolated organ bath while superfused with oxygenated synthetic interstitial fluid. The distal end of the deep vibrissal nerve was teased for single unit recordings of responses from slowly adapting mechanoreceptors to standard bending of the hair. Sinus hair type I and type II receptors could be clearly identified by their respective characteristic firing pattern. Their responses were stable for at least 5 h even if the sinus hair had been stored at 4 degrees C for 24 h beforehand. Electron microscopic examination of these hairs at the end of experiments showed well preserved ultrastructure without abnormalities. The short diffusion distances in this preparation make it well suited for studying drug effects with the aim of investigating the mechanoelectric transduction process in these receptors.
从大鼠身上分离出鼻窦毛，并在一个隔离的器官浴中进行检查，同时用含氧的合成组织液进行灌流。将深部触须神经的远端分离以记录慢适应机械感受器对毛发标准弯曲的反应的单个单位。鼻窦毛I型和II型受体可以通过它们各自独特的放电模式清楚地识别出来。即使鼻窦毛事先在4摄氏度下储存了24小时，它们的反应至少能稳定5小时。实验结束时对这些毛发的电子显微镜检查显示超微结构保存完好，没有异常。这种制备方法中较短的扩散距离使其非常适合研究药物作用，以研究这些受体中的机械电转导过程。
