Miyashiro I, Kaname T, Nakayama T, Nakamori S, Yagyu T, Monden T, Kikkawa N, Nishisho I, Muramatsu T, Monden M, Akiyama T
Department of Oncogene Research, Osaka University, Japan.
Cancer Lett. 1996 Sep 10;106(2):287-91. doi: 10.1016/0304-3835(96)04333-9.
Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transcription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated from of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.
中期因子（MK）是一种生长分化因子，最初作为视黄酸反应基因的产物被发现。使用逆转录聚合酶链反应（RT-PCR）检测了35例原发性结直肠癌手术切除标本中MK的表达。所有癌组织均表达MK。在25例癌组织中的5例中，除了全长MK外，还检测到了最近在各种人类肿瘤细胞系中发现的截短形式的MK。相比之下，在非癌组织中未检测到截短形式的MK，而在10例非癌组织中的8例中检测到了野生型形式。这些结果表明，截短形式的MK的表达可能与肿瘤发生有关。
