截短型中期因子在人类结直肠癌中的表达

Expression of truncated midkine in human colorectal cancers.

作者信息

Miyashiro I, Kaname T, Nakayama T, Nakamori S, Yagyu T, Monden T, Kikkawa N, Nishisho I, Muramatsu T, Monden M, Akiyama T

机构信息

Department of Oncogene Research, Osaka University, Japan.

出版信息

Cancer Lett. 1996 Sep 10;106(2):287-91. doi: 10.1016/0304-3835(96)04333-9.

DOI:10.1016/0304-3835(96)04333-9

PMID:8844985

Abstract

Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transcription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated from of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.

摘要

中期因子（MK）是一种生长分化因子，最初作为视黄酸反应基因的产物被发现。使用逆转录聚合酶链反应（RT-PCR）检测了35例原发性结直肠癌手术切除标本中MK的表达。所有癌组织均表达MK。在25例癌组织中的5例中，除了全长MK外，还检测到了最近在各种人类肿瘤细胞系中发现的截短形式的MK。相比之下，在非癌组织中未检测到截短形式的MK，而在10例非癌组织中的8例中检测到了野生型形式。这些结果表明，截短形式的MK的表达可能与肿瘤发生有关。