Aridome K, Takao S, Kaname T, Kadomatsu K, Natsugoe S, Kijima F, Aikou T, Muramatsu T
First Department of Surgery, Kagoshima University Faculty of Medicine, Japan.
Br J Cancer. 1998 Aug;78(4):472-7. doi: 10.1038/bjc.1998.517.
Midkine (MK) is a growth factor identified as a product of a retinoic acid-responsive gene. A truncated form of MK mRNA, which lacks a sequence encoding the N-terminally located domain, was recently found in cancer cells. We investigated the expression of the truncated MK mRNA in specimens of 47 surgically removed human gastrointestinal organs using polymerase chain reaction. Truncated MK was not detected in all of the 46 corresponding non-cancerous regions. On the other hand, this short MK mRNA was expressed in the primary tumours in 12 of 16 gastric cancers, 8 of 13 colorectal carcinomas, five of nine hepatocellular carcinomas, two of two oesophageal carcinomas and one ampullary duodenal cancer. In addition, truncated MK was detectable in all of the 14 lymph node metastases but in none of three metastatic sites in the liver, suggesting that truncated MK mRNA could become a good marker of nodal metastases in gastrointestinal tract.
中期因子（MK）是一种作为视黄酸反应基因产物而被鉴定出的生长因子。最近在癌细胞中发现了一种截短形式的MK信使核糖核酸（mRNA），它缺少编码位于N端区域的序列。我们使用聚合酶链反应研究了47个手术切除的人体胃肠道器官标本中截短的MK mRNA的表达情况。在46个相应的非癌区域中均未检测到截短的MK。另一方面，这种短的MK mRNA在16例胃癌中的12例、13例结直肠癌中的8例、9例肝细胞癌中的5例、2例食管癌中的2例以及1例壶腹十二指肠癌的原发性肿瘤中表达。此外，在所有14个淋巴结转移灶中均可检测到截短的MK，但在3个肝转移部位中均未检测到，这表明截短的MK mRNA可能成为胃肠道淋巴结转移的良好标志物。
