Miyashiro I, Kaname T, Shin E, Wakasugi E, Monden T, Takatsuka Y, Kikkawa N, Muramatsu T, Monden M, Akiyama T
Department of Oncogene Research, Osaka University, Suita, Japan.
Breast Cancer Res Treat. 1997 Mar;43(1):1-6. doi: 10.1023/a:1005748728351.
The expression of midkine (MK), a growth/differentiation factor, was assessed in 34 surgically resected specimens of primary breast cancer or mastopathy. Using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, all of the non-cancerous and cancerous tissues were found to express MK except for one breast cancer specimen. Northern blot analysis revealed that MK mRNA was also expressed in the normal breast tissues examined. Immunohistochemical analysis of the MK protein was performed on a limited number of the specimens, showing that some cancerous tissues were immunoreactive with anti-MK antibodies. Furthermore, using RT-PCR analysis, expression of not only the wild-type but also a truncated form of MK, which was recently found in various human tumor cell lines, was detected in 6 of 26 cancerous tissues but not in non-cancerous tissues.
对34例原发性乳腺癌或乳腺病手术切除标本评估生长/分化因子中期因子（MK）的表达。采用逆转录聚合酶链反应（RT-PCR）分析，发现除1例乳腺癌标本外，所有非癌组织和癌组织均表达MK。Northern印迹分析显示，在所检测的正常乳腺组织中也表达MK mRNA。对有限数量的标本进行了MK蛋白的免疫组织化学分析，结果表明一些癌组织与抗MK抗体呈免疫反应性。此外，采用RT-PCR分析，在26例癌组织中的6例中检测到不仅野生型而且最近在各种人类肿瘤细胞系中发现的截短形式的MK的表达，而在非癌组织中未检测到。
