Kubota T, Kubouchi K, Koh J, Enomoto K, Ishibiki K, Abe O
Jpn J Surg. 1983 Jul;13(4):381-4. doi: 10.1007/BF02469523.
The tumor cells (0.5 ml, 1 X 10(7) of MCF-7 line were inoculated into the subcutaneous tissue or intraperitoneum of female BALB/c nude mice. Primarily transplanted mice were treated with 17 beta-estradiol dipropionate (E2) in a dose of 5 mg/kg and 17 alpha-hydroxy progesterone caproate (Pg) in a dose of 250 mg/kg once a week. After the transferable strain was established, tumors were transplanted into female and male mice treated with E2, Pg, and E2 + Pg. The tumors treated with E2 or E2 + Pg grew exponentially while tumors in the other group regressed. Pg was assumed to play some role in the growth of MCF-7, in the presence of estrogen. Although cytosol estrogen receptors (ERc), nuclear estrogen receptors (ERn), and progesterone receptors (PgR) were detected by dextran coated-charcoal method and exchange assay in the growing tumors, ERn and PgR of regressing tumors was usually negative. This MCF-7 strain in nude mice may be a promising animal model for studying chemo-hormone therapy for human breast carcinomas.
将0.5毫升、1×10⁷个MCF - 7细胞系的肿瘤细胞接种到雌性BALB/c裸鼠的皮下组织或腹腔内。对初次移植的小鼠每周一次给予剂量为5毫克/千克的二丙酸雌二醇（E2）和剂量为250毫克/千克的己酸17α - 羟孕酮（Pg）进行治疗。在建立可移植株后，将肿瘤移植到用E2、Pg和E2 + Pg治疗的雌性和雄性小鼠体内。用E2或E2 + Pg治疗的肿瘤呈指数生长，而另一组的肿瘤则消退。假定在雌激素存在的情况下，Pg在MCF - 7的生长中起一定作用。尽管通过葡聚糖包被活性炭法和交换测定法在生长的肿瘤中检测到了胞质雌激素受体（ERc）、核雌激素受体（ERn）和孕激素受体（PgR），但消退肿瘤的ERn和PgR通常为阴性。裸鼠中的这种MCF - 7株可能是研究人类乳腺癌化学激素疗法的一个有前景的动物模型。
