Bragadóttir R, Jarkman S
Department of Ophthalmology, Linköping University, Sweden.
Doc Ophthalmol. 1995;90(3):291-303. doi: 10.1007/BF01203864.
The effects of the stable cyclic adenosine monophosphate analogue adenosine 3',5'-cyclic monophosphorothioate Sp-isomer (Sp-cAMPS) on the direct-current electroretinogram and the standing potential of the eye were studied. Corneal recordings were obtained from unilaterally vitrectomized albino rabbit eyes during alternating intravitreal perfusions with Sp-cAMPS and a control solution (Pharmacia eye irrigating solution). The contralateral eye was used as a control. To evaluate further the effects on the c-wave, in vivo intraretinal microelectrode measurements were made during simultaneous intravitreal perfusion of Sp-cAMPS and irrigating solution, respectively. Sp-cAMPS in concentrations of 1, 10 and 100 microM was tested by corneal direct-current electroretinography. There was no significant effect on the a-wave amplitude. The b-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM was tested by corneal direct-current electroretinography. There was no significant effect on the a-wave amplitude. The b-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM (p < 0.01, n = 7). The c-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100 microM (p < 0.01, n = 7). The c-wave amplitude was reversibly elevated at a concentration of 10 microM (p < 0.001, n = 8), and this effect was more pronounced at 100 microM (p < 0.001, n = 7). The SP increased reversibly at a concentration of 100 microM (p < 0.001, n = 7). Microelectrode recordings were performed with Sp-cAMPS at a concentration of 100 microM. The recordings showed significant increases in both the transepithelial potential (p < 0.01, n = 3) and the slow PIII (p < 0.01, n = 3). The effects of Sp-cAMPS on the b-wave as well as the two components of the c-wave suggest influences on both the inner retina and the retinal pigment epithelium of the rabbit eye.
研究了稳定的环磷酸腺苷类似物3',5'-环磷硫腺苷Sp-异构体（Sp-cAMPS）对直流视网膜电图和眼静息电位的影响。在对单侧玻璃体切除的白化兔眼交替进行玻璃体内灌注Sp-cAMPS和对照溶液（Pharmacia眼冲洗液）期间，从角膜进行记录。对侧眼用作对照。为了进一步评估对c波的影响，在分别同时进行玻璃体内灌注Sp-cAMPS和冲洗液期间进行了体内视网膜内微电极测量。通过角膜直流视网膜电图测试了浓度为1、10和100微摩尔的Sp-cAMPS。对a波振幅没有显著影响。通过角膜直流视网膜电图测试，在Sp-cAMPS浓度为100微摩尔时，b波振幅可逆性升高（p<0.01，n = 7）。对a波振幅没有显著影响。在Sp-cAMPS浓度为100微摩尔时，b波振幅可逆性升高（p<0.01，n = 7）。在Sp-cAMPS浓度为100微摩尔时，c波振幅可逆性升高（p<0.01，n = 7）。在浓度为10微摩尔时，c波振幅可逆性升高（p<0.001，n = 8），且在100微摩尔时这种作用更明显（p<0.001，n = 7）。在浓度为100微摩尔时，SP可逆性增加（p<0.001，n = 7）。用浓度为100微摩尔的Sp-cAMPS进行微电极记录。记录显示跨上皮电位（p<0.01，n = 3）和慢PIII（p<0.01，n = 3）均显著增加。Sp-cAMPS对b波以及c波的两个成分的影响表明其对兔眼的内视网膜和视网膜色素上皮均有影响。
