Rijke R P, Hanson W R, Plaisier H M
Cell Tissue Kinet. 1977 Jul;10(4):399-406. doi: 10.1111/j.1365-2184.1977.tb00307.x.
Epithelial cell kinetics were studied in an ileal segment after transposition to proximal jejunum. The number of cells per villus column in the transposed ileum increased after 4--7 days to reach values normal for jejunum after 14--30 days. This increase was accompanied by a simultaneous increase in the number of cells per crypt column up to 130% of values in jejunum and ileum in situ. The percentage of labelled crypt cells, after labelling with 3H-thymidine, and the relative size of the proliferative cell compartment in the crypt in the transposed ileum did not differ from values in the ileum in situ at any time interval after surgery. The total proliferative activity per crypt, which was determined by scintillation counting of isolated crypts after 3H-thymidine labelling, increased two-fold from 7 days after surgery. Cell migration studies showed that the increase in the number of villus cells was probably not caused by a change in the life span of the epithelial cells. It seems that the increase in the number of villus cells in ileal epithelium after transposition to proximal jejunum is brought about by an enlargement of the crypt, while the relative size of the proliferative cell compartment in the crypt remains unchanged.
对一段回肠段转位至空肠近端后的上皮细胞动力学进行了研究。转位回肠中每个绒毛柱的细胞数量在4 - 7天后增加，在14 - 30天后达到空肠的正常数值。这种增加伴随着每个隐窝柱细胞数量的同时增加，达到空肠和原位回肠中细胞数量的130%。用³H - 胸腺嘧啶核苷标记后，转位回肠中标记隐窝细胞的百分比以及隐窝中增殖细胞区室的相对大小在术后任何时间间隔都与原位回肠中的数值没有差异。通过对³H - 胸腺嘧啶核苷标记后分离的隐窝进行闪烁计数确定的每个隐窝的总增殖活性从术后7天起增加了两倍。细胞迁移研究表明，绒毛细胞数量的增加可能不是由上皮细胞寿命的变化引起的。似乎回肠上皮转位至空肠近端后绒毛细胞数量的增加是由隐窝扩大导致的，而隐窝中增殖细胞区室的相对大小保持不变。
