Gutierrez C, Yaun A, Cusick C G
Department of Anatomy and Neurosciences Training Program, Tulane University, New Orleans, Louisiana 70119, USA.
J Comp Neurol. 1995 Dec 25;363(4):545-562. doi: 10.1002/cne.903630404.
The architecture of the pulvinar of rhesus monkeys was investigated by acetylcholinesterase (AChE) histochemistry, and by immunocytochemistry for calbindin-D28k and the SMI-32 antibody. The presence of four inferior subdivisions, comparable to those found in architectonic-connectional studies in squirrel monkeys (C.G. Cusick, J.L. Scripter, J.G. Darensbourg, and J.T. Weber, 1993, J. Comp. Neurol. 336:1-30), provided a basis for a proposed revised terminology for visual sectors of the macaque pulvinar. In the present study, the inferior pulvinar (PI) was identified as a neurochemically distinct region that included the traditional cytoarchitectonic nucleus PI and adjacent portions of the lateral and medial pulvinar nuclei, PL and PM. In calbindin-D28k stains, the lateral subdivision of the inferior pulvinar (PIL) had less intense neuropil staining than the adjacent central division, PIC. The PIL was characterized by large, intensely immunopositive neurons seldom found within PIC. PIL occupied the traditional PL and PI and exhibited a narrow shell zone, PIL-S, restricted to PL. The medial division of the inferior pulvinar (PIM) was in a location previously shown to be strongly connected with the middle temporal visual area (MT) in macaques. PIM was found in the medial one-half of the traditional PI and extended into adjacent portions of the traditional PM and PL. PIM was distinguished by less intense neuropil staining for calbindin and many cells stained with the SMI-32 antibody for neurofilament protein. In AChE stains, PIL was moderately dark, PIC appeared lighter, and PIM was characterized by small, intensely stained patches. The small posterior division (PIP) stained darkly for calbindin, lightly for AChE, and was unstained with the SMI-32 antibody. Thus, neurochemical, and perhaps connectional, subdivisions exist within PI, the region of the pulvinar that relays information to striate, "lower order" extrastriate, and inferotemporal visual cortex.
通过乙酰胆碱酯酶（AChE）组织化学以及钙结合蛋白-D28k和SMI-32抗体的免疫细胞化学方法，对恒河猴丘脑枕的结构进行了研究。发现了四个下部分支，与松鼠猴的结构-连接研究中发现的分支类似（C.G. Cusick、J.L. Scripter、J.G. Darensbourg和J.T. Weber，1993年，《比较神经学杂志》336:1-30），这为猕猴丘脑枕视觉区域的拟议修订术语提供了基础。在本研究中，下丘脑枕（PI）被确定为一个神经化学上不同的区域，包括传统细胞构筑学上的PI核以及外侧和内侧丘脑枕核（PL和PM）的相邻部分。在钙结合蛋白-D28k染色中，下丘脑枕的外侧分支（PIL）的神经毡染色强度低于相邻的中央分支PIC。PIL的特征是在PIC内很少发现的大的、强免疫阳性神经元。PIL占据了传统的PL和PI，并表现出一个狭窄的壳区，PIL-S，仅限于PL。下丘脑枕的内侧分支（PIM）位于先前显示与猕猴颞中视觉区（MT）有强连接的位置。PIM位于传统PI的内侧一半，并延伸到传统PM和PL的相邻部分。PIM的特征是钙结合蛋白的神经毡染色强度较低，并且许多细胞被用于神经丝蛋白的SMI-32抗体染色。在AChE染色中，PIL呈中度深色，PIC显得较浅，而PIM的特征是有小的、染色深的斑块。小的后部分支（PIP）钙结合蛋白染色深，AChE染色浅，并且未被SMI-32抗体染色。因此，在PI内存在神经化学以及可能的连接细分，PI是丘脑枕中向纹状、“低阶”纹外和颞下视觉皮层传递信息的区域。
