Yousef M I, Bertheussen K, Ibrahim H Z, Helmi S, Seehy M A, Salem M H
Department of Environmental Studies, Alexandria University, Egypt.
J Environ Sci Health B. 1996 Jan;31(1):99-115. doi: 10.1080/03601239609372977.
A sensitive sperm-motility test for the evaluation of cytotoxic effects of carbofuran and glyphosate in a defined protein-free culture medium is described. The sperm motility was compared to that obtained with a protein-containing medium. The use of protein-free medium considerably increased the sensitivity of sperm cells from rabbit and human to the toxic effects of the pesticide. The respective IC50 values (the concentration needed to cause 50% inhibition of sperm motility) in protein-free medium of carbofuran and glyphosate were 321 and 48.2 microM with human sperm, and 116 and 23.5 microM with rabbit sperm. Whereas, the corresponding values in protein-containing medium were 920 and 740 microM, and 910 and 500 microM with human and rabbit sperm, respectively. Our results show that testing human and rabbit sperm in protein-free medium proves to be a more sensitive method than that in protein-containing medium. Additionally, the use of rabbit sperm is a more sensitive test system than human sperm. This study suggests that the rabbit sperm test appears to have a potential for the assessment of toxicity on human reproduction.
本文描述了一种灵敏的精子活力测试方法，用于评估在特定无蛋白培养基中呋喃丹和草甘膦的细胞毒性作用。将精子活力与在含蛋白培养基中获得的结果进行比较。使用无蛋白培养基显著提高了兔和人精子细胞对农药毒性作用的敏感性。在无蛋白培养基中，呋喃丹和草甘膦对人精子的半数抑制浓度（IC50值，即导致精子活力抑制50%所需的浓度）分别为321和48.2微摩尔，对兔精子分别为116和23.5微摩尔。而在含蛋白培养基中，相应的值分别为920和740微摩尔，以及910和500微摩尔，分别对应人精子和兔精子。我们的结果表明，在无蛋白培养基中测试人及兔精子比在含蛋白培养基中更为灵敏。此外，使用兔精子比人精子是更灵敏的测试系统。本研究表明，兔精子测试似乎具有评估对人类生殖毒性的潜力。
