Wen C, Li M, Whitworth J A
Department of Medicine, St. George Hospital, University of New South Wales, Sydney, Australia.
J Cardiovasc Pharmacol. 1996 Apr;27(4):482-6. doi: 10.1097/00005344-199604000-00005.
The objective of the present study was to validate a transonic flowmeter system with two probes (model 3SS for cardiac output (CO) and 1RB for organ flows) in Sprague-Dawley (SD) rats first by measuring blood flow through pump-infused isolated renal artery and ascending aorta, and then through measurements of CO and renal, mesenteric, and hindquarter blood flow (RBF, MBF, HBF) in vivo. We measured in vivo baseline flow and changes in flow induced by dopamine and propranolol for CO, prostaglandin E2 (PGE2), and angiotensin II (AII) for RBF and pentobarbital sodium for MBF and HBF. Correlations between meter and pump flow were linear (r = 0.999, p < 0.001) with close agreement both in ascending aorta and renal artery flow measurements. The baseline values were 15 +/- 0.7 ml/100 g/min for CO, 4 +/- 0.1 ml/100 g/min for RBF, 7 +/- 0.3 ml/100 g/min for MBF, and 6 +/- 0.3 ml/100 g/min for HBF, respectively. The system reliably detected increase and/or decrease in CO and regional blood flows. The transonic flowmeter system is accurate, highly reproducible, and compatible with other established techniques for measuring CO and regional blood flows in the rat.
本研究的目的是首先通过测量经泵灌注的离体肾动脉和升主动脉的血流，然后通过在体测量心输出量（CO）以及肾、肠系膜和后肢血流量（RBF、MBF、HBF），来验证一种带有两个探头的跨音速流量计系统（用于测量心输出量的3SS型和用于测量器官血流量的1RB型）在Sprague-Dawley（SD）大鼠中的性能。我们测量了在体基线血流以及多巴胺和普萘洛尔诱导的CO血流变化、前列腺素E2（PGE2）和血管紧张素II（AII）诱导的RBF血流变化以及戊巴比妥钠诱导的MBF和HBF血流变化。流量计与泵流量之间的相关性呈线性（r = 0.999，p < 0.001），在升主动脉和肾动脉血流测量中均具有高度一致性。CO的基线值分别为15±0.7 ml/100 g/min，RBF为4±0.1 ml/100 g/min，MBF为7±0.3 ml/100 g/min，HBF为6±0.3 ml/100 g/min。该系统能够可靠地检测到CO和局部血流量的增加和/或减少。跨音速流量计系统准确、高度可重复，并且与其他已确立的测量大鼠CO和局部血流量的技术兼容。
