Tani A, Tatsumi E, Nakamura F, Kumagai S, Kosaka Y, Sano K, Nakamura H, Amakawa R, Ohno H
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Leukemia. 1996 Oct;10(10):1592-603.
An Epstein-Barr virus (EBV)-negative Burkitt's lymphoma (BL) cell line, designated Black93, was established in culture from a patient who developed acute tumor lysis syndrome (ATLS). Growth inhibition in vitro by dexamethasone (DXM) and the expression of bcl-2 protein (Bcl-2) were investigated in Black93 and 17 other cell lines derived from EBV-negative or -positive BL, pre-B acute lymphoblastic leukemia (ALL), follicular lymphoma (FL), and EBV-positive lymphoblastoid cell lines of normal B cell origin (B-LCL), assuming an inherent susceptibility of Black93 to cell death. The most marked growth inhibition by DXM was observed in Black93, two other BL, two pre-B-ALL and two FL lines. The other cell lines were less sensitive or were resistant. DNA extracted from the Black93 cells treated with DXM showed a ladder of oligo-nucleosomal DNA on electrophoresis. On testing of fixed smears by indirect immunofluorescence, bcl-2 protein (Bcl-2) was undetectable in Black93 and three BL lines but was detected in all the other cell lines at varying intensity. Western blot analysis showed mostly the same results. In the BL lines, the most DXM-sensitive cell lines lacked Bcl-2 expression, and the DXM-resistant cell lines always expressed Bcl-2. While none of the DXM-resistant cell lines lacked Bcl-2 expression, several pre-B or FL lines that expressed [correction of expessed] Bcl-2 were sensitive to DXM. Black93 is the first reported cell line established from a patient with ATLS. The positive sensitivity to DXM and the lack of Bcl-2 expression observed in Black93 are a major characteristic exhibited frequently by BL lines and, probably, by fresh BL cells. These properties may contribute to the precipitation of ATLS.
一株爱泼斯坦-巴尔病毒（EBV）阴性的伯基特淋巴瘤（BL）细胞系，命名为Black93，是从一名发生急性肿瘤溶解综合征（ATLS）的患者体内培养建立的。研究了地塞米松（DXM）对Black93以及另外17株源自EBV阴性或阳性BL、前B急性淋巴细胞白血病（ALL）、滤泡性淋巴瘤（FL）和正常B细胞来源的EBV阳性淋巴母细胞系（B-LCL）的体外生长抑制作用，以及bcl-2蛋白（Bcl-2）的表达情况，假定Black93对细胞死亡具有内在易感性。在Black93、另外两株BL、两株前B-ALL和两株FL细胞系中观察到DXM对其生长的抑制作用最为显著。其他细胞系则不太敏感或具有抗性。用DXM处理的Black93细胞提取的DNA在电泳时显示出寡核小体DNA梯状条带。通过间接免疫荧光检测固定涂片发现，Black93和三株BL细胞系中未检测到bcl-2蛋白（Bcl-2），但在所有其他细胞系中均检测到不同强度的Bcl-2。蛋白质免疫印迹分析显示了大致相同的结果。在BL细胞系中，对DXM最敏感的细胞系缺乏Bcl-2表达，而对DXM有抗性的细胞系总是表达Bcl-2。虽然对DXM有抗性的细胞系均未缺乏Bcl-2表达，但一些表达Bcl-2的前B或FL细胞系对DXM敏感。Black93是首个报道的从ATLS患者体内建立的细胞系。在Black93中观察到的对DXM的阳性敏感性以及缺乏Bcl-2表达是BL细胞系以及可能新鲜的BL细胞经常表现出的主要特征。这些特性可能导致ATLS的发生。
