Satoh T, Sakai N, Kubo T, Enokido Y, Uchiyama Y, Hatanaka H
Institute for Protein Research, Osaka University, Japan.
Neurosci Lett. 1995 Dec 8;201(2):119-22. doi: 10.1016/0304-3940(95)12150-1.
To investigate the mechanism of serum deprivation-induced apoptosis in PC12 cells, we performed flow cytometry with the viable cell-specific fluorogen, fluorescein diacetate (FDA). Intact PC12 cells were positive when stained with FDA, and those cells were identical with the major population which possess larger forward and side scattered light. Apoptotic PC12 cells decreased the forward and side scatter light associated with the loss of FDA-positive cells. Nerve growth factor (NGF) and the bcl-2 protein protected the cells from serum deprivation-induced apoptosis. These data suggested that the process of apoptosis in PC12 cells can be analyzed with flow cytometry.
为了研究血清剥夺诱导PC12细胞凋亡的机制，我们使用活细胞特异性荧光染料二乙酸荧光素（FDA）进行了流式细胞术检测。完整的PC12细胞用FDA染色时呈阳性，这些细胞与具有较大前向和侧向散射光的主要群体相同。凋亡的PC12细胞前向和侧向散射光减少，这与FDA阳性细胞的丢失有关。神经生长因子（NGF）和bcl-2蛋白可保护细胞免受血清剥夺诱导的凋亡。这些数据表明，PC12细胞的凋亡过程可用流式细胞术进行分析。
