Hinton R H, Mullock B M
Clin Chim Acta. 1977 Jul 1;78(1):159-62. doi: 10.1016/0009-8981(77)90349-7.
The major bile proteins can be detected by two-dimensional electrophoresis in the serum of cholestatic rats, three days after ligation of the common bile duct. The novel electrophoresis system used agarose gel electrophoresis in the first dimension followed by equilibrium electrophoresis in a polyacrylamide gel gradient.
在胆总管结扎三天后的胆汁淤积大鼠血清中,主要胆汁蛋白可通过二维电泳检测到。所采用的新型电泳系统,第一维使用琼脂糖凝胶电泳,随后在聚丙烯酰胺凝胶梯度中进行平衡电泳。