Gossler K, Schaller K H, Zschiesche W
Clin Chim Acta. 1977 Jul 1;78(1):91-101. doi: 10.1016/0009-8981(77)90341-2.
A simple method to determine hippuric acid in urine by high pressure liquid chromatography is described. An ion exchange column is used for the separation. The detection is carried out with a photometer. The analytical criteria of the reliability of the method are tested. The results for the precision and the accuracy of the method meet the guidelines of the statistical quality control. 34 urinary constituents were tested for their interference in order to examine the specificity. Measuring urine samples from 46 normal persons yielded hippuric acid concentrations between 160 and 2010 mg/1. The median was 400 mg/1, equivalent to 357 mg/g creatinine. These results are compared to those given in the literature. Comparative investigations of those urine samples by a gas chromatographic method matched well, with a coefficient of regression of 0.86.
描述了一种用高压液相色谱法测定尿中马尿酸的简单方法。采用离子交换柱进行分离，用光度计进行检测。测试了该方法可靠性的分析标准。该方法的精密度和准确度结果符合统计质量控制指南。检测了34种尿成分的干扰情况以检验特异性。对46名正常人的尿样进行检测，马尿酸浓度在160至2010mg/1之间。中位数为400mg/1，相当于357mg/g肌酐。将这些结果与文献中的结果进行了比较。用气相色谱法对那些尿样进行的对比研究结果吻合良好，回归系数为0.86。
