采用高效液相色谱法定量测定尿液中的马尿酸和间甲基马尿酸。
Quantitative determination of hippuric and m-methylhippuric acids in urine by high-speed liquid chromatography.
作者信息
Matsui H, Kasao M, Imamura S
出版信息
Br J Ind Med. 1977 Nov;34(4):310-3. doi: 10.1136/oem.34.4.310.
High-speed liquid chromatography employing an ultraviolet photometric detector has been applied to the simultaneous determination of hippuric and m-methylhippuric acids in urine. Reversed-phase partition chromatography is carried out on a muBondapak C18 column with methanol-water as the eluent system. This method obviates the necessity for isolation or reaction of these acids before assay. The only pretreatment necessary is extraction of sample with ethyl acetate. A linear relationship is obtained between the peak heights and the hippuric or m-methylhippuric acid concentrations. Mean recovery of hippuric and m-methylhippuric acids in urine is 99.8% and 99.3% respectively. The determination of hippuric acid by this method gives lower concentrations in normal urine than does the colorimetric method of Umberger and Fiorese (1963).
采用紫外光度检测器的高速液相色谱法已用于同时测定尿中马尿酸和间甲基马尿酸。在μBondapak C18柱上以甲醇 - 水为洗脱剂系统进行反相分配色谱。该方法无需在测定前对这些酸进行分离或反应。唯一必要的预处理是用乙酸乙酯萃取样品。峰高与马尿酸或间甲基马尿酸浓度之间呈线性关系。尿中马尿酸和间甲基马尿酸的平均回收率分别为99.8%和99.3%。用该方法测定正常尿中马尿酸的浓度比Umberger和Fiorese（1963年）的比色法测得的浓度低。
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