Yang Y D
Analytical and Testing Center, Hebei Agrotechnical Teachers College, Changli, Peoples Republic of China.
Biomed Chromatogr. 1998 Mar-Apr;12(2):47-9. doi: 10.1002/(SICI)1099-0801(199803/04)12:2<47::AID-BMC717>3.0.CO;2-Y.
A simple method for the simultaneous determination of creatine, uric acid, creatinine and hippuric acid in urine using reversed-phase high performance liquid chromatography was described. Chromatography was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a mobile phase of 0.02 mol/L KH2PO4, and UV detection at 220 nm. The recoveries of creatine, uric acid, creatinine and hippuric acid were 103.13, 99.86, 98.26 and 98.24%, respectively. The calibration curves were linear within the concentration range of 2-200 micrograms/mL for creatine, uric acid and hippuric acid, and 2-400 micrograms/mL for creatinine. The detection limits of the method were 0.69, 0.21, 0.10 and 0.095 microgram/mL for creatine, uric acid, creatinine and hippuric acid, respectively. This method has been applied to the analysis of urine samples from normal subjects and some patients.
描述了一种使用反相高效液相色谱法同时测定尿液中肌酸、尿酸、肌酐和马尿酸的简单方法。在Nova-Pak C18（3.9 x 150 mm）柱上进行色谱分析，流动相为0.02 mol/L KH2PO4，并在220 nm处进行紫外检测。肌酸、尿酸、肌酐和马尿酸的回收率分别为103.13%、99.86%、98.26%和98.24%。肌酸、尿酸和马尿酸在2 - 200微克/毫升的浓度范围内校准曲线呈线性，肌酐在2 - 400微克/毫升的浓度范围内校准曲线呈线性。该方法对肌酸、尿酸、肌酐和马尿酸的检测限分别为0.69、0.21、0.10和0.095微克/毫升。该方法已应用于正常受试者和一些患者尿液样本的分析。
