McCarthy CM, Murray L
Department of Biology, 3AF, New Mexico State University, Las Cruces, New Mexico 88003 USA.
Microb Ecol. 1996 Nov;32(3):305-21. doi: 10.1007/BF00183065.
The quantitation and characterization of indigenous bacteria of a deep aquifer, located in the southwestern United States and contaminated with halogenated aliphatic compounds, was undertaken. Water samples were obtained aseptically from depths of 45 to 151 m from four sites that ranged from 260 to 1,800 m in distance from the location of contaminant release. Sediment samples were also obtained from the proximal and distal sites for analyses. Results for aerobic and anaerobic colony-forming units were obtained on four agar media that were used to retrieve heterotrophs, oligotrophs, and pseudomonads. Most probable number estimates were obtained from a liquid medium favorable for oligotrophs. Representative isolates were tested against Biolog plates (Biolog, Inc., Hayward, Calif.) for patterns of carbon source utilization. Of 103 Gram-negative (GN) isolates, 48 could not be identified and the others were only tentatively identified via the Biolog database, and none of the 35 Gram-positive (GP) isolates were identifiable. However, the metabolic patterns were subjected to average cluster linkage analyses; the GN and GP bacteria were separable into eight and four groups, respectively. The oligotroph group comprised one-third of the GN and one-half of the GP isolates. The consensus carbon source utilization pattern for each group was determined and will be useful in future characterization of additional aquifer bacterial isolates. Although predominantly aerobic and oligotrophic, the microbial community of this aquifer was highly diverse with discernible viability and metabolic features of the microbiota distinctive to each of the four water and two sediment samples.
对位于美国西南部且被卤代脂肪族化合物污染的深层含水层中的本土细菌进行了定量和特性分析。从距离污染物释放点260至1800米的四个地点，无菌采集了深度为45至151米的水样。还从近端和远端地点采集了沉积物样本用于分析。在用于获取异养菌、贫养菌和假单胞菌的四种琼脂培养基上获得了需氧和厌氧菌落形成单位的结果。从有利于贫养菌的液体培养基中获得了最可能数估计值。对代表性分离株进行了Biolog平板（Biolog公司，加利福尼亚州海沃德）测试，以检测碳源利用模式。在103株革兰氏阴性（GN）分离株中，48株无法鉴定，其他分离株仅通过Biolog数据库初步鉴定，35株革兰氏阳性（GP）分离株均无法鉴定。然而，对代谢模式进行了平均聚类连锁分析；GN和GP细菌分别可分为八组和四组。贫养菌群分别占GN分离株的三分之一和GP分离株的二分之一。确定了每组的一致碳源利用模式，这将有助于未来对其他含水层细菌分离株进行特性分析。尽管该含水层的微生物群落主要为需氧和贫养型，但高度多样化，四个水样和两个沉积物样本中的微生物群具有明显的活力和独特的代谢特征。
