Muroi H, Kubo I
Department of Environmental Science, Policy and Management, University of California, Berkeley 94720-3112, USA.
J Appl Bacteriol. 1996 Apr;80(4):387-94. doi: 10.1111/j.1365-2672.1996.tb03233.x.
The inhibitory and bactericidal activities of anacardic acid and totarol, alone and in combination with methicillin, were investigated against methicillin-resistant Staphylococcus aureus (MRSA). The growth of two MRSA strains was inhibited by 6 x 25 microg ml-1 of anacardic acid and 0 x 78 microg ml-1 of totarol. The time-kill curve study showed that these two compounds were bactericidal against MRSA. Anacardic acid killed MRSA cells more rapidly than totarol, and no viable cells were detected after being exposed to 6 x 25 microg ml-1 of anacardic acid for 6 h. Anacardic acid showed bactericidal activity against MRSA at any stage of growth, and also even when cell division was inhibited by chloramphenicol. In the combination studies, the minimal inhibitory concentration (MIC) of methicillin was lowered from 800 to 1 x 56 microg ml-1 for MRSA ATCC 33591, and from 800 to 6 x 25 microg ml-1 for MRSA ATCC 33592, by combining with 1/2 x MIC of anacardic acid. The time-kill curves demonstrated synergistic bactericidal activities for these combinations.
研究了没食子酸和托酚酮单独及与甲氧西林联合使用时对耐甲氧西林金黄色葡萄球菌（MRSA）的抑制和杀菌活性。没食子酸6×25微克/毫升和托酚酮0×78微克/毫升可抑制两种MRSA菌株的生长。时间-杀菌曲线研究表明，这两种化合物对MRSA具有杀菌作用。没食子酸杀灭MRSA细胞的速度比托酚酮更快，在暴露于6×25微克/毫升没食子酸6小时后未检测到活细胞。没食子酸在MRSA生长的任何阶段均显示出杀菌活性，即使在氯霉素抑制细胞分裂时也是如此。在联合研究中，对于MRSA ATCC 33591，与1/2×MIC的没食子酸联合使用时，甲氧西林的最低抑菌浓度（MIC）从800微克/毫升降至1×56微克/毫升，对于MRSA ATCC 33592，从800微克/毫升降至6×25微克/毫升。时间-杀菌曲线证明这些联合用药具有协同杀菌活性。
