Barchiesi F, Hollis R J, Messer S A, Scalise G, Rinaldi M G, Pfaller M A
Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750, USA.
Diagn Microbiol Infect Dis. 1995 Nov;23(3):99-103. doi: 10.1016/0732-8893(95)00169-7.
Electrophoretic karyotype (EK) was used to type 13 clinical isolates of Cryptococcus neoformans from eight AIDS patients. All of the isolates were also tested for their in vitro susceptibilities to fluconazole, itraconazole, D0870, flucytosine, and amphotericin B by a broth macrodilution technique performed according to the National Committee for Clinical Laboratory Standards recommendations. Although all strains were isolated from a limited geographic area, DNA typing showed a wide genetic variation in this group of patients, yielding seven different patterns. Two patients in whom C. neoformans was isolated in the same time period shared similar EK profiles, suggesting the possibility of cross-infection. In three patients, sequential isolates were evaluated: in two of them, EK analysis showed the persistence of the same genotype throughout the infection, whereas from the third, two isolates of C. neoformans with two different DNA profiles were obtained. Despite the small number of strains considered in this study, our susceptibility data indicate that C. neoformans isolates are very susceptible to the new triazoles.
采用电泳核型分析（EK）技术对来自8例艾滋病患者的13株新型隐球菌临床分离株进行分型。所有分离株还按照美国国家临床实验室标准委员会的建议，采用肉汤稀释法进行了对氟康唑、伊曲康唑、D0870、氟胞嘧啶和两性霉素B的体外药敏试验。尽管所有菌株均分离自有限的地理区域，但DNA分型显示这组患者存在广泛的基因变异，产生了7种不同的模式。在同一时期分离出新型隐球菌的2例患者具有相似的EK图谱，提示存在交叉感染的可能性。对3例患者的连续分离株进行了评估：其中2例患者的EK分析显示在整个感染过程中基因型持续存在，而第3例患者获得了2株具有不同DNA图谱的新型隐球菌分离株。尽管本研究纳入的菌株数量较少，但我们的药敏数据表明新型隐球菌分离株对新型三唑类药物非常敏感。
