Kirkpatrick W R, McAtee R K, Revankar S G, Fothergill A W, McCarthy D I, Rinaldi M G, Patterson T F
Department of Medicine, University of Texas Health Science Center at San Antonio, 78284-7881, USA.
J Clin Microbiol. 1998 May;36(5):1330-2. doi: 10.1128/JCM.36.5.1330-1332.1998.
A simple screening method for fluconazole susceptibility of Cryptococcus neoformans using 2% dextrose Sabouraud dextrose agar (SabDex) with fluconazole was compared to the National Committee for Clinical Laboratory Standards (NCCLS) broth macrodilution method. By this method, fluconazole-susceptible C. neoformans isolates are significantly smaller on medium with fluconazole than on fluconazole-free medium. Isolates with decreased susceptibility have normal-size colonies on medium containing fluconazole. The 48-h NCCLS broth macrodilution MICs (NCCLS MICs) for isolates with normal-size colonies on 8- or 16-microg/ml fluconazole plates were predicted to be > or =8 or > or =16 microg/ml, respectively. On medium with 16 microg of fluconazole per ml, all strains (84 of 84) for which the NCCLS MICs were <16 microg/ml were correctly predicted, as were all isolates (7 of 7) for which the MICs were > or =16 microg/ml. Agar dilution appears to be an effective screening method for fluconazole resistance in C. neoformans.
使用含氟康唑的2%葡萄糖沙氏葡萄糖琼脂（SabDex）对新型隐球菌的氟康唑敏感性进行简单筛选方法，并与美国国家临床实验室标准委员会（NCCLS）肉汤稀释法进行比较。通过这种方法，对氟康唑敏感的新型隐球菌分离株在含氟康唑的培养基上比在不含氟康唑的培养基上明显更小。敏感性降低的分离株在含氟康唑的培养基上具有正常大小的菌落。在8或16μg/ml氟康唑平板上具有正常大小菌落的分离株的48小时NCCLS肉汤稀释MIC（NCCLS MIC）预计分别>或 =8或>或 =16μg/ml。在每毫升含16μg氟康唑的培养基上，所有NCCLS MIC<16μg/ml的菌株（84株中的84株）以及所有MIC>或 =16μg/ml的分离株（7株中的7株）均被正确预测。琼脂稀释法似乎是一种有效的新型隐球菌氟康唑耐药性筛选方法。
