Hartung M, Stahl A
Cytogenet Cell Genet. 1977;18(6):309-19. doi: 10.1159/000130777.
In the embryonic mouse ovary, studies of meiotic onset reveal the existence of a preleptotene stage of chromosome condensation. This stage begins with the appearance of very fine and irregular filaments, which gather around the chromocenters constituted by centromeric heterochromatin. At maximal condensation the nucleus contains 40 compact chromosomal masses. Decondensation engenders the reappearance of filaments more dense and regular than those seen at the onset of the condensation stage. The filaments elongate while progressively taking on the appearance of leptotene-stage chromosomes. The oocytes at different phases of condensation stage represent 41% of the germ cels in the 13-day-old mouse embryo. This proportion reaches 63% at 14 days and then declines to 3% at 17 days. Spectrophotometric studies of the condensation-stage nucleus, following staining by the Feulgen reaction, indicated that the nucleus contains 4C DNA. This observation, together with the morphologic characteristics of this stage, suggests that the oocyte is already engaged in prophase. Time studies indicate that the condensation stage occurs immediately prior to leptotene.
