Saparbaev M, Prakash L, Prakash S
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555-1061, USA.
Genetics. 1996 Mar;142(3):727-36. doi: 10.1093/genetics/142.3.727.
The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for nucleotide excision repair and they also act in mitotic recombination. The Rad1-Rad10 complex has a single-stranded DNA endonuclease activity. Here, we show that the mismatch repair genes MSH2 and MSH3 function in mitotic recombination. For both his3 and his4 duplications, and for homologous integration of a linear DNA fragment into the genome, the msh3 delta mutation has an effect on recombination similar to that of the rad1 delta and rad10 delta mutations. The msh2 delta mutation also reduces the rate of recombination of the his3 duplication and lowers the incidence of homologous integration of a linear DNA fragment. Epistasis analyses indicate that MSH2 and MSH3 function in the RAD1-RAD10 recombination pathway, and studies presented here suggest an involvement of the RAD1-RAD10 pathway in reciprocal recombination. The possible roles of Msh2, Msh3, Rad1, and Rad10 proteins in genetic recombination are discussed. Coupling of mismatch binding proteins with the recombinational machinery could be important for ensuring genetic fidelity in the recombination process.
酿酒酵母的RAD1和RAD10基因是核苷酸切除修复所必需的，它们也在有丝分裂重组中发挥作用。Rad1-Rad10复合物具有单链DNA内切酶活性。在这里，我们表明错配修复基因MSH2和MSH3在有丝分裂重组中起作用。对于his3和his4重复，以及线性DNA片段整合到基因组中的同源整合，msh3δ突变对重组的影响与rad1δ和rad10δ突变相似。msh2δ突变也降低了his3重复的重组率，并降低了线性DNA片段同源整合的发生率。上位性分析表明MSH2和MSH3在RAD1-RAD10重组途径中起作用，本文提出的研究表明RAD1-RAD10途径参与了相互重组。讨论了Msh2、Msh3、Rad1和Rad10蛋白在基因重组中的可能作用。错配结合蛋白与重组机制的耦合对于确保重组过程中的遗传保真度可能很重要。
