Zamora J, Riedemann S, Cabezas X, Vega S
Instituto de Microbiología, Universidad Austral de Chile, Valdivia.
Rev Latinoam Microbiol. 1995 Jul-Sep;37(3):267-72.
Kidneys of six different species of 93 wild rodents captured in the rural area of Valdivia (Chile) were simultaneously examined for leptospira infection by means of Levaditi silver stain, dark ground microscopy in wet smears, and immunofluorescence and immunoperoxidase techniques using pooled antiserum against hardjo and pomona serovars. Leptospira was shown to be present in 40 (43.0%) rodents. They were detected in five of the six species: Akodon olivaceus, Akodon longipilis, Rattus rattus, Oryzomis longicaudatus and Mus musculus. Levaditi's technique detected the highest number of positive samples (67.5%) and the dark field microscopy the lowest (32.5%). The detection of leptospira in the kidneys of these wild rodents suggests that their urine may play an important role in the dissemination of leptospirosis in this area of Valdivia.
对在智利瓦尔迪维亚农村地区捕获的93只6种不同野生啮齿动物的肾脏，同时采用莱瓦迪蒂银染色法、湿涂片暗视野显微镜检查以及使用针对哈焦和波摩那血清型的混合抗血清的免疫荧光和免疫过氧化物酶技术，检测钩端螺旋体感染情况。结果显示，40只（43.0%）啮齿动物体内存在钩端螺旋体。在6种动物中的5种检测到了钩端螺旋体，分别是：橄榄阿氏鼠、长毛阿氏鼠、黑家鼠、长须稻鼠和小家鼠。莱瓦迪蒂技术检测到的阳性样本数量最多（67.5%），暗视野显微镜检测到的数量最少（32.5%）。在这些野生啮齿动物肾脏中检测到钩端螺旋体表明，它们的尿液可能在瓦尔迪维亚这一地区钩端螺旋体病的传播中起重要作用。
