Thomas R
Biotics Limited, Surrey, UK.
Folia Microbiol (Praha). 1995;40(1):4-16. doi: 10.1007/BF02816523.
In Prague in 1964, the participants in the International Congress on Antibiotics collectively presented an authoritative account of the existing understanding of the mechanism of biosynthesis of many of the major groups of antibiotics and other microbial metabolites. This information was derived to a large extent from applications of the then comparatively novel radioisotopic technique for studying the mode incorporation of labelled precursors. The intervening 30 years has witnessed remarkable progress in techniques for manipulating microbial genes and enzymes, while at the chemical level, advances in spectroscopic and chromatographic procedures have facilitated the use of stable isotopes as direct probes for the small scale exploration of metabolic pathways. As a consequence, our understanding of many mechanistic details has been considerably enhanced. Some of the developments to date will be reviewed, with particular reference to the elaboration of biosynthetic pathways leading to many of the antibiotics and other natural products described at the earlier Prague symposium.
1964年在布拉格，国际抗生素大会的与会者共同发表了一份权威性报告，阐述了当时对许多主要抗生素和其他微生物代谢产物生物合成机制的理解。这些信息在很大程度上源自当时相对新颖的放射性同位素技术在研究标记前体掺入模式方面的应用。在随后的30年里，操纵微生物基因和酶的技术取得了显著进展，而在化学层面，光谱和色谱技术的进步促进了稳定同位素作为代谢途径小规模探索直接探针的应用。因此，我们对许多机制细节的理解有了显著增强。本文将回顾一些迄今为止的进展，尤其会涉及到导致早期布拉格研讨会上所描述的许多抗生素和其他天然产物的生物合成途径的阐述。
