Smith C, Habermann E, Hamerman D
J Rheumatol. 1979 Mar-Apr;6(2):147-55.
Antisera to rheumatoid and nonrheumatoid synovial cells were prepared by: intraarticular injection of rabbits with these cells, subsequent synovectomy, and autologous immunization of each animal with the cultured rabbit synovial cells, or homogenized whole rabbit synovial membrane. Resultant antisera were studied by immunofluorescence and 51chromium cytotoxicity tests against other cultured rheumatoid and nonrheumatoid cells. Activity of positive sera could be removed by absorption with nonrheumatoid cells, except for one antiserum which appeared to produce immunofluorescent staining specific for rheumatoid cells.
向兔关节腔内注射这些细胞，随后进行滑膜切除术，并用培养的兔滑膜细胞或匀浆的全兔滑膜对每只动物进行自体免疫。通过免疫荧光和针对其他培养的类风湿性和非类风湿性细胞的51铬细胞毒性试验对所得抗血清进行研究。阳性血清的活性可通过用非类风湿性细胞吸收而去除，但有一种抗血清似乎产生了对类风湿性细胞特异的免疫荧光染色。
