Sloan T B, Martincic R R, Anderson B
Exp Cell Biol. 1981;49(4):195-206. doi: 10.1159/000163823.
A number of antisera were produced in rabbits immunized with cultured synovial tissue-derived cells. The synovial cells were injected utilizing a variety of immune manipulations in order to elicit different antibody specificities and to enhance antibodies that may differentiate antigens of rheumatoid arthritic and nonrheumatoid arthritic synovial tissue-derived cells. The major sources of antigens common to both rheumatoid and nonrheumatoid cells were cross-reactive with normal human serum, nonrheumatoid synovial fluids and fetal calf serum components. Adsorptions with the latter antigen sources yielded different antisera with greater differential reactivities to either rheumatoid or nonrheumatoid synovial cells. Crossed immunoelectrophoretic analyses of extracts of the synovial cells using unabsorbed antisera differentiated five electrophoretically distinct antigens with various quantitative differences of the antigens being noted between the rheumatoid and nonrheumatoid extracts. The results suggest that several antigens are quantitatively differentially expressed on rheumatoid-derived synovial cell strains compared to synovial strains of nonrheumatoid origin.
用培养的滑膜组织来源细胞免疫兔子，制备了多种抗血清。利用多种免疫操作注射滑膜细胞，以引发不同的抗体特异性，并增强可能区分类风湿性关节炎和非类风湿性关节炎滑膜组织来源细胞抗原的抗体。类风湿性和非类风湿性细胞共有的主要抗原来源与正常人血清、非类风湿性滑液和胎牛血清成分发生交叉反应。用后一种抗原来源进行吸附，产生了对类风湿性或非类风湿性滑膜细胞具有更大差异反应性的不同抗血清。使用未吸收的抗血清对滑膜细胞提取物进行交叉免疫电泳分析，区分出五种电泳上不同的抗原，类风湿性和非类风湿性提取物之间的抗原存在各种定量差异。结果表明，与非类风湿性来源的滑膜细胞系相比，类风湿性来源的滑膜细胞系上几种抗原的表达在数量上存在差异。
