Mody V D, Satia M C, Gandhi T P, Modi I A, Modi R I, Chakravarthy B K
R&D Centre, Cadila Labs. Ltd, Ahmedabad, India.
J Chromatogr B Biomed Appl. 1996 Feb 9;676(1):175-9. doi: 10.1016/0378-4347(95)00377-0.
A high-performance thin-layer chromatographic procedure has been developed for the determination of ranitidine, a H2-receptor antagonist, in plasma. The detection and quantification were performed without using internal standards. A single-stage extraction procedure was followed for extracting ranitidine from plasma, and a known amount of the extract was spotted on precoated silica gel F254 plates. Ranitidine was quantified using a Shimadzu CS930 dual-wavelength TLC scanner. The method provides a direct estimate of total ranitidine present in the plasma.
已开发出一种高效薄层色谱法，用于测定血浆中H2受体拮抗剂雷尼替丁。检测和定量过程无需使用内标。采用单步萃取法从血浆中提取雷尼替丁，并将已知量的提取物点样于预涂硅胶F254板上。使用岛津CS930双波长TLC扫描仪对雷尼替丁进行定量。该方法可直接估算血浆中雷尼替丁的总量。
