Wada S, Kajihara H, Murakami H, Sueda T, Matsuura Y
First Department of Surgery, Hiroshima University, School of Medicine, Japan.
Artif Organs. 1996 Aug;20(8):930-5. doi: 10.1111/j.1525-1594.1996.tb04572.x.
Fluosol-DA and FC43 emulsion were developed as red blood cell substitutes. However, these perfluorochemical (PFC)-based compounds are not very promising because of their immunosuppressive effects. Therefore we used these compounds as immunosuppressive agents in guinea pig-to-rat discordant heart xenotransplantations. In rats treated with these compounds (5 microliters/g body weight), rhythmic beating of the hearts was maintained for 265.7 +/- 50.3 min (Fluosol-DA-treated group) and 424.3 +/- 40.1 min (FC43 emulsion-treated group), respectively whereas the untreated heartbeat lasted for only 15.5 +/- 6.6 min. In the xenografted heart treated with PFC compounds, no pathologic changes, such as multiple coronary thromboses due to hyperacute rejection, had occurred by 3-4 h after reperfusion, and the endothelial cells were well preserved. We conclude that these PFC compounds can prolong the survival time of guinea pig heart xenografts by inhibiting thrombus formation in the xenografted heart.
氟碳代血液（Fluosol-DA）和FC43乳剂被开发用作红细胞替代物。然而，这些基于全氟化合物（PFC）的物质由于其免疫抑制作用而不太有前景。因此，我们将这些化合物用作豚鼠到大鼠异种异位心脏移植中的免疫抑制剂。在用这些化合物（5微升/克体重）处理的大鼠中，心脏的有节律跳动分别维持了265.7±50.3分钟（氟碳代血液处理组）和424.3±40.1分钟（FC43乳剂处理组），而未处理的心脏仅跳动了15.5±6.6分钟。在用PFC化合物处理的异种移植心脏中，再灌注后3 - 4小时未出现诸如因超急性排斥导致的多发性冠状动脉血栓形成等病理变化，并且内皮细胞保存良好。我们得出结论，这些PFC化合物可通过抑制异种移植心脏中的血栓形成来延长豚鼠心脏异种移植物的存活时间。
