吉姆萨细胞学检查与实时聚合酶链反应技术在刮片涂片诊断皮肤利什曼病中的比较

Comparison of cytologic giemsa and real-time polymerase chain reaction technique for the diagnosis of cutaneous leishmaniasis on scraping smears.

作者信息

Meymandi Simin Shamsi, Bahmanyar Mohammad, Dabiri Shahriar, Aflatonian Mohammad Reza, Bahmanyar Shahram, Meymandi Manzumeh Shamsi

机构信息

Kerman Leishmaniasis Research Center, Department of Dermatology, Afzalipour Medical School, Kerman, Iran.

出版信息

Acta Cytol. 2010 Jul-Aug;54(4):539-45. doi: 10.1159/000325174.

DOI:10.1159/000325174

PMID:20715653

Abstract

OBJECTIVE

To compare the results of real-time polymerase chain reaction (RT-PCR) for detection of Leishmania DNA in Giemsa-stained skin scraping slides with direct microscopic evaluation of Giemsa-stained skin scrapings and to estimate the sensitivity and specificity of RT-PCR.

STUDY DESIGN

We used 30 samples from cases diagnosed with cutaneous leishmaniasis (CL), 16 from clinically suspected individuals but negative in direct microscopic evaluation and 50 normal individuals from nonendemic dry type CL areas.

RESULTS

All samples of CL positive and 8 of suspected cases were positive for RT-PCR, and all nonleishmaniasis cases were negative. The sensitivity and specificity of RT-PCR were 100% (95% CI 88-100%) and 88% (95% CI 78-95%), respectively. We also found an inverse association between the number of lympnocytes (OR = 0.90, 95% CI 0.83-0.97%), neutrophils and Leishman bodies.

摘要

目的

比较实时聚合酶链反应（RT-PCR）检测吉姆萨染色皮肤刮片载玻片上利什曼原虫DNA的结果与吉姆萨染色皮肤刮片的直接显微镜评估结果，并评估RT-PCR的敏感性和特异性。

研究设计

我们使用了30份来自确诊为皮肤利什曼病（CL）病例的样本、16份来自临床疑似个体但直接显微镜评估为阴性的样本以及50份来自非流行干燥型CL地区的正常个体样本。

结果

所有CL阳性样本和8例疑似病例的RT-PCR结果均为阳性，所有非利什曼病病例均为阴性。RT-PCR的敏感性和特异性分别为100%（95%可信区间88-100%）和88%（95%可信区间78-95%）。我们还发现淋巴细胞数量（比值比=0.90，95%可信区间0.83-0.97%）、中性粒细胞与利什曼小体之间呈负相关。