Uttenthal A, Jensen N P, Blom J Y
Danish Veterinary Laboratory, Copenhagen V, Denmark.
Vet Rec. 1996 Aug 3;139(5):114-7. doi: 10.1136/vr.139.5.114.
Ten outbreaks of calf respiratory disease in Danish dairy herds were investigated by lung lavage, and in eight of the herds paired blood samples were tested serologically. In six of the 10 herds bovine respiratory syncytial virus (BRSV) antigen was detected in the lung lavage fluids. In only one calf was coronavirus and BRSV detected simultaneously. The paired blood samples confirmed that four of the herds were BRSV-infected, and in one herd the BRSV infection was diagnosed by the paired blood samples alone. Significant increases in antibody titres against coronavirus were observed in two herds, both in combination with other virological agents. No adenovirus antigen was detected in any of the lavage samples, but in two herds a significant increase in antibody titres against adenovirus was observed. Parainfluenza-3 (PI-3) virus was not detected in the lung lavage fluids, and in four of the herds, no antibodies to PI-3 were detected. In three herds no viral involvement could be found. The findings suggest that BRSV may be an important causative agent in calf respiratory disease in Denmark, even in very young calves.
通过肺灌洗对丹麦奶牛场的10起犊牛呼吸道疾病暴发进行了调查，并且在其中8个牛群中对配对的血液样本进行了血清学检测。在10个牛群中的6个，在肺灌洗液中检测到了牛呼吸道合胞病毒（BRSV）抗原。仅在1头犊牛中同时检测到了冠状病毒和BRSV。配对的血液样本证实其中4个牛群感染了BRSV，并且在1个牛群中仅通过配对的血液样本就诊断出了BRSV感染。在2个牛群中观察到针对冠状病毒的抗体滴度显著升高，这两个牛群均同时感染了其他病毒病原体。在任何灌洗样本中均未检测到腺病毒抗原，但在2个牛群中观察到针对腺病毒的抗体滴度显著升高。在肺灌洗液中未检测到副流感3型（PI-3）病毒，并且在4个牛群中未检测到针对PI-3的抗体。在3个牛群中未发现病毒感染。这些发现表明，BRSV可能是丹麦犊牛呼吸道疾病的一个重要病原体，即使在非常年幼的犊牛中也是如此。
