Haque Q M, Sugiyama A, Iwade Y, Midorikawa Y, Yamauchi T
Department of Public Health, School of Medicine, Mie University, Mie-Ken, Japan.
Curr Microbiol. 1996 May;32(5):239-45. doi: 10.1007/s002849900043.
Diarrheal and environmental isolates of 39 strains of Aeromonas spp. were studied for detection of virulence factors. Although these 39 strains did not produce either heat-labile or heat-stable enterotoxins, culture filtrates of 31 strains produced cytopathic effects on Vero cells. Among these, culture filtrates of three strains of Aeromonas hydrophila and one strain of Aeromonas caviae could be neutralized by Escherichia coli O157:H7 Shiga-like toxin 1 antiserum. A single band of plasmid DNA of 2.14 kbp was isolated from these strains of Aeromonas spp. and E. coli O157:H7, which could be amplified by the polymerase chain reaction (PCR), employing oligonucleotide primers from the Shiga-like toxin 1 (SLT1) gene of E. coli O157:H7. E. coli HB 101 cells when transformed with the same plasmid showed cytopathic effects on Vero cells, which indicates that the SLT 1 homolog gene(s) of Aeromonas spp. is plasmid encoded. These results suggest that Aeromonas spp. may also produce Shiga-like toxin 1, or at least a cytotoxin with some homology with the Shiga-like toxin 1 of E. coli O157:H7.
对39株气单胞菌属细菌的腹泻和环境分离株进行了毒力因子检测研究。虽然这39株细菌既不产生不耐热肠毒素也不产生耐热肠毒素，但31株细菌的培养滤液对Vero细胞产生了细胞病变效应。其中，3株嗜水气单胞菌和1株豚鼠气单胞菌的培养滤液可被大肠杆菌O157:H7志贺样毒素1抗血清中和。从这些气单胞菌属细菌和大肠杆菌O157:H7菌株中分离出一条2.14 kbp的质粒DNA条带，使用来自大肠杆菌O157:H7志贺样毒素1（SLT1）基因的寡核苷酸引物，通过聚合酶链反应（PCR）可对其进行扩增。用相同质粒转化的大肠杆菌HB 101细胞对Vero细胞产生了细胞病变效应，这表明气单胞菌属细菌的SLT 1同源基因是由质粒编码的。这些结果表明，气单胞菌属细菌可能也产生志贺样毒素1，或至少产生一种与大肠杆菌O157:H7志贺样毒素1有一定同源性的细胞毒素。
