Salla S, Redbrake C, Frantz A
Department of Ophthalmology, RWTH Aachen, Germany.
Graefes Arch Clin Exp Ophthalmol. 1996 Aug;234(8):521-6. doi: 10.1007/BF00184862.
Quantification of adenosine phosphates in human corneal extracts has been performed using spectrophotometry. We employed the bioluminescence technique to obtain a more sensitive assay for adenosine phosphates and to reduce the volume of the test sample.
The bioluminescence assay for ATP, already known from sterility control, was modified and expanded. Standard curves were established using a standard solution with equimolar concentrations of ATP, ADP and AMP. To monitor the method, adenosine phosphates were measured in 35 human corneal extracts using both spectrophometry and bioluminescence.
Linear standard curves ranging from 1 to 45 pmol were established. The two methods yielded comparable results despite the use of a basic dilution of 1:100 for the new technique.
Bioluminescence provides a highly sensitive quantification of adenosine phosphates in the human cornea and facilitates an extremely detailed evaluation of the metabolic status of the cornea.
已使用分光光度法对人角膜提取物中的腺苷磷酸进行定量。我们采用生物发光技术来获得对腺苷磷酸更灵敏的检测方法，并减少测试样品的体积。
对已用于无菌控制的ATP生物发光检测方法进行了改进和扩展。使用含有等摩尔浓度ATP、ADP和AMP的标准溶液建立标准曲线。为监测该方法，使用分光光度法和生物发光法对35份人角膜提取物中的腺苷磷酸进行了测量。
建立了范围为1至45皮摩尔的线性标准曲线。尽管新技术使用了1:100的基本稀释度，但两种方法得出了可比的结果。
生物发光提供了对人角膜中腺苷磷酸的高灵敏度定量，并有助于对角膜代谢状态进行极其详细的评估。
