Chandra T S, Shethna Y I
J Bacteriol. 1977 Aug;131(2):389-98. doi: 10.1128/jb.131.2.389-398.1977.
Thiobacillus novellus was able to grow with oxalate, formate, formamide, and methanol as sole sources of carbon and energy. Extensive growth on methanol required yeast extract or vitamins. Glyoxylate carboligase was detected in extracts of oxalate-grown cells. Ribulose bisphosphate carboxylase was found in extracts of cells grown on formate, formamide, and thiosulfate. These data indicate that oxalate is utilized heterotrophically in the glycerate pathway, and formate and formamide are utilized autotrophically in the ribulose bisphosphate pathway. Nicotinamide adenine dinucleotide-linked formate dehydrogenase was present in extracts of oxalate-, formate-, formamide-, and methanol-grown cells but was absent in thiosulfate- and acetate-grown cells.
新型硫杆菌能够以草酸盐、甲酸盐、甲酰胺和甲醇作为唯一的碳源和能源进行生长。在甲醇上大量生长需要酵母提取物或维生素。在以草酸盐生长的细胞提取物中检测到乙醛酸羧化酶。在以甲酸盐、甲酰胺和硫代硫酸盐生长的细胞提取物中发现了核酮糖二磷酸羧化酶。这些数据表明，草酸盐在甘油酸途径中被异养利用，而甲酸盐和甲酰胺在核酮糖二磷酸途径中被自养利用。烟酰胺腺嘌呤二核苷酸连接的甲酸脱氢酶存在于以草酸盐、甲酸盐、甲酰胺和甲醇生长的细胞提取物中，但在以硫代硫酸盐和乙酸盐生长的细胞中不存在。
