Gordon P M, Visich P S, Goss F L, Fowler S, Warty V, Denys B J, Metz K F, Robertson J
Human Energy Research Laboratory, University of Pittsburgh, USA.
Int J Sports Med. 1996 Jul;17(5):332-7. doi: 10.1055/s-2007-972856.
In order to compare the influence of a single bout of exercise on HDL-C metabolism with normal variability, 12 male runners (mean age: 24.9 +/- 4 yr) who ran 15-30 miles per week underwent exercise (E) and control (C) experimental conditions. During the E trial subjects ran on a motor driven treadmill at 75% (42.5 +/- 4.7 ml.kg-1.min-1) VO2max until 800 Kcals were expended. The C trial consisted of no exercise. Subjects were instructed to follow the same diet and keep a four d food diary during each experimental condition. Fasted blood samples were obtained at the same time of day in each condition at time points corresponding to 24 h pre-exercise (24 PRE), 6 h post- (6 h) and 24 h post-exercise (24 h). Plasma was analyzed for HDL-C, HDL2-C and HDL3-C (mg.dl-1). In addition post-heparin plasma samples were analyzed for lipoprotein lipase (LPL) and hepatic lipase (HL) activity (mumol.FFA-1.ml-1). All values were adjusted for changes in plasma volume and compared to Baseline. HDL-C levels were unaltered following the C trial. However, following the E trial, HDL-C increased (p < 0.01) above baseline values at 24 h. The increase in HDL-C was reflected in the HDL3-C subfraction (p < 0.05). Analysis of lipolytic activity revealed an overall greater LPL activity (p < 0.05) in the E trial vs the C trial. In addition, a decrease in HL was observed at 24 h (p < 0.05) but was not different between experimental conditions. These data suggest that exercise and not normal variability are responsible for alterations in lipolytic activity and corresponding increases in HDL-C levels.
为了比较单次运动对高密度脂蛋白胆固醇（HDL-C）代谢的影响与正常变异性，12名每周跑15 - 30英里的男性跑步者（平均年龄：24.9±4岁）接受了运动（E）和对照（C）实验条件。在E试验中，受试者在电动跑步机上以75%（42.5±4.7毫升·千克⁻¹·分钟⁻¹）的最大摄氧量（VO2max）跑步，直到消耗800千卡热量。C试验包括不运动。受试者被要求在每个实验条件下遵循相同的饮食并记录四天的食物日记。在每种条件下，于一天中的同一时间点采集空腹血样，对应运动前24小时（24 PRE）、运动后6小时（6 h）和运动后24小时（24 h）。分析血浆中的HDL-C、HDL2-C和HDL3-C（毫克·分升⁻¹）。此外，分析肝素化后血浆样本中的脂蛋白脂肪酶（LPL）和肝脂肪酶（HL）活性（微摩尔·游离脂肪酸⁻¹·毫升⁻¹）。所有值均针对血浆容量变化进行调整，并与基线进行比较。C试验后HDL-C水平未改变。然而，在E试验后，HDL-C在24小时时高于基线值（p < 0.01）。HDL-C的增加反映在HDL3-C亚组分中（p < 0.05）。脂解活性分析显示，与C试验相比，E试验中LPL活性总体上更高（p < 0.05）。此外，在24小时时观察到HL下降（p < 0.05），但在不同实验条件之间无差异。这些数据表明，是运动而非正常变异性导致了脂解活性的改变以及HDL-C水平的相应升高。
