Prentice M J, Farrant J
J Clin Microbiol. 1977 Jul;6(1):4-9. doi: 10.1128/jcm.6.1.4-9.1977.
Factors influencing the survival of chlamydiae after freezing were reexamined. From the data presented, it is suggested that preservation of laboratory-grown chlamydiae is best achieved through the use of sucrose as the cryoprotective agent, in the presence of 10% serum. Dimethyl sulfoxide and glycerol are more toxic. The period of exposure to sucrose before freezing must be kept as short as possible and be at 4 degrees C rather than at room temperature. The rate of cooling during freezing in sucrose is not important; however, cooling at a rate slower than 1 degrees C/min should be avoided. Since chlamydial survival is increased by rapid thawing, the volume of the sample should be kept to a minimum. Thawed suspensions should be inoculated onto cell monolayers without delay. The application of these methods may increase the proportion of stored clinical specimens in which chlamydiae can be found.
对影响衣原体冷冻后存活的因素进行了重新研究。根据所呈现的数据，建议在10%血清存在的情况下，使用蔗糖作为冷冻保护剂来实现实验室培养衣原体的最佳保存。二甲基亚砜和甘油毒性更大。冷冻前接触蔗糖的时间必须尽可能短，且应在4℃而非室温下进行。在蔗糖中冷冻期间的冷却速度并不重要；然而，应避免以低于1℃/分钟的速度冷却。由于快速解冻可提高衣原体的存活率，样本体积应保持最小。解冻后的悬液应立即接种到细胞单层上。应用这些方法可能会增加可检测到衣原体的储存临床标本的比例。
