Tjiam K H, van Heijst B Y, de Roo J C, de Beer A, van Joost T, Michel M F, Stolz E
Br J Vener Dis. 1984 Apr;60(2):92-4. doi: 10.1136/sti.60.2.92.
We compared the survival of a laboratory strain of Chlamydia trachomatis serovar L-2 in different media and at different temperatures (room temperature, 4 degrees C, and -70 degrees C). At these temperatures the best storage medium was 2SP (0.2 mol/l sucrose in 0.02 mol/l phosphate buffer supplemented with 10% fetal calf serum). We used material obtained from patients to study the sensitivity of the culture method as a function of sample storage time and temperature. Compared with results on direct inoculation, material stored in 2SP for 48 hours gave 11% fewer positive cultures at 4 degrees C and 14% fewer at room temperature. Of samples which gave negative results on direct inoculation, 4% were positive after storage at 4 degrees C for 48 hours and 2% after storage at -70 degrees C for a week. As expected, the number of inclusion forming units in the original material proved to be important for the percentage of positive cultures among the stored samples.
我们比较了沙眼衣原体L-2血清型实验室菌株在不同培养基以及不同温度（室温、4℃和-70℃）下的存活情况。在这些温度条件下，最佳保存培养基是2SP（0.02 mol/L磷酸盐缓冲液中含0.2 mol/L蔗糖，并添加10%胎牛血清）。我们使用从患者获取的材料来研究培养方法的敏感性与样本保存时间和温度的关系。与直接接种的结果相比，保存在2SP中48小时的材料在4℃时阳性培养物减少了11%，在室温下减少了14%。直接接种呈阴性结果的样本中，4%在4℃保存48小时后呈阳性，2%在-70℃保存一周后呈阳性。正如预期的那样，原始材料中包涵体形成单位的数量对于保存样本中阳性培养物的百分比至关重要。
