Ladds G, Rasmussen E M, Young T, Nielsen O, Davey J
School of Biochemistry, University of Birmingham, UK.
Mol Microbiol. 1996 Apr;20(1):35-42. doi: 10.1111/j.1365-2958.1996.tb02486.x.
Haploid cells of the fission yeast Schizosaccharomyces pombe exist in one of two mating types, referred to as M and P. Conjugation occurs between cells of opposite mating type and is controlled by the reciprocal action of diffusible pheromones. Loss of function of the sxa2 gene in M cells causes hypersensitivity to the P-factor mating pheromone and a reduction in mating efficiency. Here we demonstrate the secretion of an sxa2-dependent carboxypeptidase that inactivates P-factor by removal of the C-terminal leucine residue.
裂殖酵母粟酒裂殖酵母的单倍体细胞存在两种交配型之一，称为M型和P型。相反交配型的细胞之间会发生接合，这由可扩散信息素的相互作用控制。M细胞中sxa2基因功能丧失会导致对P因子交配信息素高度敏感，且交配效率降低。在此，我们证明了一种依赖sxa2的羧肽酶的分泌，该羧肽酶通过去除C端亮氨酸残基使P因子失活。
