Seike Taisuke, Sakata Natsue, Matsuda Fumio, Furusawa Chikara
Center for Biosystems Dynamics Research, RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Fungi (Basel). 2021 Apr 29;7(5):350. doi: 10.3390/jof7050350.
The fission yeast , comprising var. and var. varieties, has unique characteristics such as striking hyphal growth not seen in other species; however, information on its diversity and evolution, in particular mating and sporulation, remains limited. Here we compared the growth and mating phenotypes of 17 wild strains of , including eight var. strains newly isolated from an insect (). Unlike existing wild strains isolated from fruits/plants, the strains isolated from sporulated at high frequency even under nitrogen-abundant conditions. In addition, one of the strains from was stained by iodine vapor, although the type strain of var. is not stained. Sequence analysis further showed that the nucleotide and amino acid sequences of pheromone-related genes have diversified among the eight strains from , suggesting crossing between cells of different genetic backgrounds occurs frequently in this insect. Much of yeast ecology remains unclear, but our findings suggest that insects such as might be a good niche for mating and sporulation, and will provide a basis for the understanding of sporulation mechanisms via signal transduction, as well as the ecology and evolution of yeast.
裂殖酵母包括变种和变种，具有独特的特征，如在其他物种中未见的显著菌丝生长；然而，关于其多样性和进化的信息，特别是交配和孢子形成方面，仍然有限。在这里，我们比较了17种野生裂殖酵母菌株的生长和交配表型，其中包括从一种昆虫（）新分离出的8种变种菌株。与从水果/植物中分离出的现有野生菌株不同，从昆虫中分离出的菌株即使在氮充足的条件下也能高频产孢。此外，尽管变种的模式菌株不会被碘蒸气染色，但来自昆虫的其中一个菌株会被碘蒸气染色。序列分析进一步表明，在来自昆虫的8个菌株中，信息素相关基因的核苷酸和氨基酸序列已经多样化，这表明在这种昆虫中，不同遗传背景的裂殖酵母细胞之间经常发生杂交。酵母生态学的许多方面仍不清楚，但我们的研究结果表明，诸如昆虫等可能是交配和孢子形成的良好生态位，并将为通过信号转导理解孢子形成机制以及酵母的生态学和进化提供基础。
