Williams T W, Lyons J M, Braude A I
J Immunol. 1977 Aug;119(2):671-4.
Polymorphonuclear (PMN) leukocytes from acute pyelonephritic exudates of rats were examined for cytoxicity against various target cells. Pyelonephritic PMN leukocytes caused lysis in vitro of syngeneic renal epithelial cells in 24 to 48 hr. They also caused lysis of allogeneic and xenogeneic target cells. The susceptibility to PMN-induced cytolysis was different among various target cell lines. Cell lysis proceeded at low ratios of effector to target cells and was not dependent on the addition of antibody or lectins. PMN isolated from peritoneal exudates (starch or endotoxin induced) were also cytoxic for the target cells. Cell-free supernatants from pyelonephritic PMN-target cell mixtures, or pyelonephritic PMN leukocytes cultured alone contained cytolytic substances which were nondialyzable and heat stable Finally, the urine of pyelonephritic rats was cytotoxic. These results suggest that PMN leukocytes and their cytotoxic mediators are directly involved in the pathogenesis of renal injury in pyelonephritis.
对来自大鼠急性肾盂肾炎渗出液的多形核（PMN）白细胞进行了针对各种靶细胞的细胞毒性检测。肾盂肾炎的PMN白细胞在24至48小时内可在体外导致同基因肾上皮细胞裂解。它们还会导致异基因和异种靶细胞裂解。不同靶细胞系对PMN诱导的细胞溶解的敏感性不同。细胞裂解在效应细胞与靶细胞比例较低时即可发生，且不依赖于抗体或凝集素的添加。从腹膜渗出液（淀粉或内毒素诱导）中分离出的PMN对靶细胞也具有细胞毒性。肾盂肾炎PMN - 靶细胞混合物的无细胞上清液，或单独培养的肾盂肾炎PMN白细胞含有不可透析且热稳定的细胞溶解物质。最后，肾盂肾炎大鼠的尿液具有细胞毒性。这些结果表明，PMN白细胞及其细胞毒性介质直接参与了肾盂肾炎肾损伤的发病机制。
