Elomari M, Coroler L, Hoste B, Gillis M, Izard D, Leclerc H
Laboratoire de Bactériologie-Hygiène, Faculté de Médecine Henri Warembourg, Lille, France.
Int J Syst Bacteriol. 1996 Oct;46(4):1138-44. doi: 10.1099/00207713-46-4-1138.
The taxonomic position of eight strains isolated from mineral water and previously grouped in the authentic pseudomonads on the basis of a phenotypic analysis (cluster Ib of M. Elomari, L. Coroler, D. Izard, and H. Leclerc [J. Appl. Bacteriol. 78:71-81, 1995]) has been further studied by DNA-DNA hybridizations. Using the S1 nuclease method at 60 degrees C and labeled reference DNA from a representative strain, CFML 92-134, we showed that members of cluster Ib constituted a homogeneous group with a relative binding ratio of greater than 80% and changes in melting temperature of less than 1 degree C. With a total of 67 strains representing known or partially characterized species of the genus Pseudomonas, only 4 to 47% DNA hybridization and changes in melting temperature of between 8 and 20 degrees C were found, the highest hybridization values being measured with members of the saprophytic fluorescent pseudomonads. Since cluster Ib could also be clearly differentiated from members of the latter group and from other phenotypic clusters containing isolates from mineral water, we designated the Ib strains members of a new Pseudomonas species for which the name Pseudomonas veronii sp. nov. has been proposed. Members of this species grew on alpha-aminobutyrate, sucrose, butyrate, isobutyrate, erythritol, L-tryptophan, and trigonelline as sole sources of carbon and energy. The average G+C content of the DNA of the eight strains of P. veronii was 61.5 +/- 0.5 mol%. The type strain is CFML 92-134T (CIP 104663T), with a G+C content of 61 mol%. The clinical significance of P. veronii is unknown.
对从矿泉水中分离出的8株菌株进行了进一步研究，这些菌株先前基于表型分析被归类为正宗假单胞菌（M. Elomari、L. Coroler、D. Izard和H. Leclerc的聚类Ib [《应用细菌学杂志》78:71 - 81, 1995]）。通过DNA - DNA杂交技术，在60℃使用S1核酸酶法并以来自代表性菌株CFML 92 - 134的标记参考DNA进行研究，结果表明聚类Ib的成员构成一个同源组，相对结合率大于80%，解链温度变化小于1℃。在总共67株代表假单胞菌属已知或部分特征化物种的菌株中，仅发现4%至47%的DNA杂交，解链温度变化在8至20℃之间，与腐生荧光假单胞菌成员的杂交值最高。由于聚类Ib也能与后一组的成员以及其他包含矿泉水中分离株的表型聚类明显区分开来，我们将Ib菌株指定为一个新的假单胞菌物种的成员，并提议将其命名为维罗纳假单胞菌（Pseudomonas veronii sp. nov.）。该物种的成员能够以α - 氨基丁酸盐、蔗糖、丁酸盐、异丁酸盐、赤藓糖醇、L - 色氨酸和胡芦巴碱作为唯一碳源和能源生长。维罗纳假单胞菌8株菌株的DNA平均G + C含量为61.5±0.5 mol%。模式菌株是CFML 92 - 134T（CIP 104663T），G + C含量为61 mol%。维罗纳假单胞菌的临床意义尚不清楚。
