Verhille S, Baïda N, Dabboussi F, Hamze M, Izard D, Leclerc H
Service de Bactériologie-Hygiène, Faculté de Médecine Henri Warembourg (pôle recherche), Verdun, Lille, France.
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1559-72. doi: 10.1099/00207713-49-4-1559.
Twenty-five non-identified fluorescent Pseudomonas strains isolated from natural mineral waters were previously clustered into three phenotypic subclusters, XIIIb, XVa and XVc. These strains were characterized genotypically in the present study. DNA-DNA hybridization results and DNA base composition analysis revealed that these strains were members of two new species, for which the names Pseudomonas gessardii sp. nov. (type strain CIP 105469T) and Pseudomonas migulae sp. nov. (type strain CIP 105470T) are proposed. P. gessardii included 13 strains from phenotypic subclusters XVa and XVc. P. migulae included 10 strains from phenotypic subcluster XIIIb. The levels of DNA-DNA relatedness ranged from 71 to 100% for P. gessardii and from 74 to 100% for P. migulae. The G + C content of the DNA of each type strain was 58 mol%. DNA similarity levels, measured with 67 reference strains of Pseudomonas species, were below 55%, with delta Tm values of 13 degrees C or more. The two new species presented basic morphological characteristics common to all pseudomonads. Various phenotypic features were found to differentiate them: P. gessardii strains utilized L-arabitol, myo-inositol, adonitol, xylitol and meso-erythritol as carbon sources, whereas P. migulae strains assimilated L-arabinose, D-xylose, D-saccharate, meso-tartrate, tricarballylate, D-glucuronate, D-galacturonate, phenylacetate and histamine. The complete 16S rRNA sequences of each type strain were determined and compared with those of the type strains of Pseudomonas species. Finally, a phylogenetic tree was inferred from sequence analysis and demonstrated that the two new species fell into the 'Pseudomonas fluorescens intrageneric cluster'. To date, their clinical significance is unknown.
先前从天然矿泉水中分离出的25株未鉴定的荧光假单胞菌菌株被聚类为三个表型亚群，即XIIIb、XVa和XVc。在本研究中对这些菌株进行了基因型特征分析。DNA-DNA杂交结果和DNA碱基组成分析表明，这些菌株属于两个新物种，为此提出了新种名杰氏假单胞菌（Pseudomonas gessardii sp. nov.）（模式菌株CIP 105469T）和米氏假单胞菌（Pseudomonas migulae sp. nov.）（模式菌株CIP 105470T）。杰氏假单胞菌包括来自表型亚群XVa和XVc的13株菌株。米氏假单胞菌包括来自表型亚群XIIIb的10株菌株。杰氏假单胞菌的DNA-DNA相关性水平在71%至100%之间，米氏假单胞菌的DNA-DNA相关性水平在74%至100%之间。每个模式菌株的DNA的G + C含量为58 mol%。与67株假单胞菌属参考菌株测得的DNA相似性水平低于55%，ΔTm值为13℃或更高。这两个新物种呈现出所有假单胞菌共有的基本形态特征。发现了各种表型特征来区分它们：杰氏假单胞菌菌株利用L-阿拉伯糖醇、肌醇、阿东糖醇、木糖醇和内消旋赤藓醇作为碳源，而米氏假单胞菌菌株同化L-阿拉伯糖、D-木糖、D-糖二酸、内消旋酒石酸、三羧甲基丙烷、D-葡糖醛酸、D-半乳糖醛酸、苯乙酸和组胺。测定了每个模式菌株的完整16S rRNA序列，并与假单胞菌属物种的模式菌株的序列进行了比较。最后，从序列分析推断出系统发育树，表明这两个新物种属于“荧光假单胞菌属内聚类群”。迄今为止，它们的临床意义尚不清楚。
