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尤尔图梭状芽孢杆菌新种,一种与嗜氢产甲烷细菌以互营关系氧化乙酸的嗜温细菌。

Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogenotrophic methanogenic bacterium.

作者信息

Schnurer A, Schink B, Svensson B H

机构信息

Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.

出版信息

Int J Syst Bacteriol. 1996 Oct;46(4):1145-52. doi: 10.1099/00207713-46-4-1145.

DOI:10.1099/00207713-46-4-1145
PMID:8863449
Abstract

A syntrophic acetate-oxidizing bacterium, strain BST (T = type strain), was isolated from a previously described mesophilic triculture that was able to syntrophically oxidize acetate and form methane in stoichiometric amounts. Strain BST was isolated with substrates typically utilized by homoacetogenic bacteria. Strain BST was a spore-forming, gram-positive, rod-shaped organism which utilized formate, glucose, ethylene glycol, cysteine, betaine, and pyruvate. Acetate and sometimes formate were the main fermentation products. Small amounts of alanine were also produced from glucose, betaine, and cysteine. Strain BST grew optimally at 37 degrees C and pH 7. The G+C content of the DNA of strain BST was 32 mol%. A 16S rRNA sequence analysis revealed that strain BST was a member of a new species of the genus Clostridium. We propose the name Clostridium ultunense for this organism; strain BS is the type strain of C. ultunense.

摘要

从先前描述的嗜温三元培养物中分离出一株互营乙酸氧化细菌,菌株BST(T = 模式菌株),该三元培养物能够以互营方式氧化乙酸并按化学计量比生成甲烷。菌株BST是利用同型产乙酸细菌通常利用的底物分离得到的。菌株BST是一种形成芽孢、革兰氏阳性、杆状的微生物,利用甲酸、葡萄糖、乙二醇、半胱氨酸、甜菜碱和丙酮酸。乙酸盐以及有时还有甲酸盐是主要的发酵产物。由葡萄糖、甜菜碱和半胱氨酸也会产生少量丙氨酸。菌株BST在37℃和pH 7条件下生长最佳。菌株BST的DNA的G+C含量为32 mol%。16S rRNA序列分析表明,菌株BST是梭菌属一个新物种的成员。我们提议将该微生物命名为乌尔图梭菌;菌株BS是乌尔图梭菌的模式菌株。

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