Hayashi K, Thabit G, Bogdanske J J, Mascio L N, Markel M D
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
Arthroscopy. 1996 Aug;12(4):474-81. doi: 10.1016/s0749-8063(96)90043-2.
This study was designed to evaluate the effect of laser energy at nonablative levels on the ultrastructure of joint capsular collagen. The femoropatellar joint capsules of six mature New Zealand white rabbits were harvested immediately after death. Specimens were divided into three treatment groups (5, 10, and 15 watts) and one control group. Laser energy was applied using a holmium: YAG laser. Transmission electron microscopy showed significant ultrastructural alterations in collagenous architecture for all laser treatment groups, with increased fibril cross-sectional diameter for each of the treated groups. The fibrils began to lose their distinct edges and their periodical cross-striations at subsequently higher energy densities. A morphometric analysis showed that each subsequently higher laser energy caused a significant increase in collagen fibril diameter. Ultrastructural alteration of collagen fibril architecture caused by the thermal effect of laser energy is probably the dominant mechanism of laser-induced tissue shrinkage.
本研究旨在评估非消融水平的激光能量对关节囊胶原超微结构的影响。六只成年新西兰白兔的髌股关节囊在死后立即采集。样本分为三个治疗组（5瓦、10瓦和15瓦）和一个对照组。使用钬：钇铝石榴石激光施加激光能量。透射电子显微镜显示，所有激光治疗组的胶原结构均有明显的超微结构改变，各治疗组的纤维横截面直径均增加。在随后更高的能量密度下，纤维开始失去其清晰的边缘和周期性横纹。形态计量分析表明，随后每增加一次激光能量，胶原纤维直径就会显著增加。激光能量的热效应引起的胶原纤维结构超微结构改变可能是激光诱导组织收缩的主要机制。
